Alice Clubbs Coldron
Professor Patrick Eyers and Professor Jane Endicott
I am a second year PhD student studying at the University of Liverpool. I studied Biochemistry at the University of Leeds then came straight to Liverpool to do my PhD after graduating. During my undergraduate research project I studied the protein kinase Aurora A and its interaction with the onco-protein N-Myc, which sparked my interest in kinases. This project sparked my interest in the biochemical nature of kinases and I hence why I chose to do a PhD studying them. As well as studying the biochemical properties of kinases and their regulation, I am also interested in how protein kinases and phosphorylation impacts cell biology.
CDK18-dependent regulation of the DNA-damage response and its regulation by histidine phosphorylation
Through their activation and deactivation, cyclin dependent kinases (CDKs) are involved in the regulation of the cell cycle and the checkpoints within the cell cycle, a fundamental biological process. Some of the CDKs, such as CDK18 and CDK19 have been found to be histidine phosphorylated. Histidine phosphorylation has remained largely unexplored in mammalian cell signalling due to the instability of the phosphoramidate bond at high temperatures and low pH. For my PhD project, I am investigating the mechanisms by which protein kinases regulate these processes, to help further understand the DNA damage response and to try and uncover novel phosphorylation targets and transcriptional pathways regulated by the CDK18 and CDK19. This is a joint project with Newcastle University which combines techniques in phosphoproteomics and protein kinase biology to expand research into cellular biomarkers of DNA damage and bring about new industrial opportunities in the future.