About

Gemma Hardman

Institute of Integrative Biology Biosciences Building University of Liverpool Crown Street Liverpool L69 7ZB

Supervisor(s):

Dr Claire Eyers

I completed my undergraduate degree in Chemistry (MChem with a Year in Industry) at the University of York in 2013. The fourth year of my degree was spent on an industrial placement at Bristol Myers Squibb, a global biopharmaceutical company, where I investigated the degradation of a drug for the treatment of Type 2 diabetes, primarily using liquid chromatography (LC) and mass spectrometry (MS) techniques.
In October 2013 I began the MRes year of the BBSRC DTP programme at the University of Liverpool. I am working within the proteomics group, extending my knowledge and practical skills in LC-MS to the analysis of peptides. 

Project title: Development of LC-MS-based strategies for the analysis of histidine phosphorylation

The extent of histidine phosphorylation in mammalian systems is largely uncharacterized, particularly in comparison to serine, threonine and tyrosine phosphorylation. The major problem associated with phosphohistidine analysis is the instability of phosphorylated histidine at low pH, typical conditions for assessing protein phosphorylation either by traditional biochemical or analytical chemical methods, i.e. mass spectrometry. The purpose of this project is to develop reagents and MS-based methodologies to overcome some of the challenges associated with detection and characterisation of histidine phosphorylation in proteins from cell extracts. The first year has focused on development of suitable enrichment techniques for histidine-phosphorylated peptides.

Other Activities

I attended a Biochemical Society run Quantitative Proteomics training course and a Conference Quantitative Proteomics (ProteoMMX 3.0) in Chester in March 2014.

Publications

Maria-Belen Gonzalez-Sanchez, Francesco Lanucara, Gemma E. Hardman and Claire E. Eyers. Gas-Phase Intermolecular Phosphate Transfer within a Phosphohistidine Phosphopeptide Dimer. International Journal of Mass Spectrometry (2014) in press



Where did I get my PIPs

PIPS Internship Organisation Name

The Queen Elizabeth Prize for Engineering

Location

London

When deciding on your internship, what did you want to experience and what did you hope to gain from that experience? 

I wanted to experience a role outside of the lab.  I wanted to stretch myself and do something I had little experience in, with the hope of developing new skills.  In particular I hoped this internship would give me the opportunity to learn more about media and public engagement which are becoming increasingly important for scientists and an area I am especially interested in.

Did you get the experience you were expecting and did you achieve the personal development you had hoped to make?

My internship was certainly very different to being in the lab!  I have experienced lots of things that I would never have had exposure to during my PhD otherwise.  I have learnt a lot about engaging the public in STEM (specifically engineering but it’s applicable across the sciences), especially realising the role businesses and their early-career employees must have in this process to inspire the next generation.  Participating in the organisation of a large event also allowed me to develop my organisational skills, problem-solving abilities, teamwork (both within my small team and with the other departments also alongside the external PR company we engaged with) and gain exposure to financial planning and budgets.

Did you discover anything about yourself or make any achievements that you were not expecting? 

I discovered I can be more confident and resourceful than I originally thought.  Talking to senior engineers from around the world and having to solve problems quickly in the run up to (and during) the big event was really eye-opening and I feel I coped surprisingly well in what could have been quite stressful situations.  Being asked to write a blog post was also a steep learning curve.  After many years of writing in a formal scientific style, having to write an informal blog post about a scientific/engineering topic that was accessible for the general public was quite challenging.  However, I enjoyed the challenge and I was proud of the final result.

Has the internship made you feel differently about potential career options and has it helped to put the skills from research into a broader context?

Being away from the lab and doing something that is not particularly related to science has made me realise that I definitely want to continue in a scientific field in the future. I have enjoyed some of the ‘non-lab’ aspects of the job but I have missed not engaging with people about science and the research I do.  However, by doing an internship that has been so different from my PhD/lab-work I can see how the skills I have learnt so far in my PhD would help me in any career I chose to do in the future. Working with an organisation that plays a role in engaging and inspiring the general public and the younger generation about engineering has made me think about how I can combine this sort of outreach/engagement about science with my day-to-day PhD work.




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